abstract：:A method for the determination of metanephrine (MN; also known as metadrenaline), normetanephrine (NMN; also known as normetadrenaline) and 3-methoxytyramine (3-MT) in human urine using high-performance liquid chromatography followed by electrochemical detection (ECD) was validated primarily by comparing the results w...

journal_title：Annals of clinical biochemistry

authors： Willemsen JJ,Ross HA,Wolthers BG,Sweep CG,Kema IP

更新日期：2001-11-01 00:00:00

abstract：:Despite sonographic detection of foetal goitre, uncertainty persists in the initial diagnosis of thyrotoxicosis and hypothyroidism. The aim of this study was to establish foetal and neonatal iodothyronine and thyrotrophin reference values for the ACS-180SE analyser. From 22 to 36 weeks of gestation, median foetal seru...

journal_title：Annals of clinical biochemistry

authors： Guibourdenche J,Noël M,Chevenne D,Vuillard E,Voluménie JL,Polak M,Boissinot C,Porquet D,Luton D

更新日期：2001-09-01 00:00:00

abstract：:The ischaemic lactate-ammonia test is widely used for investigating patients with muscle pain and fatigue. It involves measuring plasma lactate and ammonia produced as a result of forearm exercise under ischaemic conditions in a fasted subject. Its clinical use is to screen patients with muscle complaints for disorder...

journal_title：Annals of clinical biochemistry

authors： Livingstone C,Chinnery PF,Turnbull DM

更新日期：2001-07-01 00:00:00

abstract：:A group of international experts prepared two lists of drugs with their serum/plasma and urine concentrations, which should be used when evaluating the performance of a new laboratory method. The two lists were verified by running in vitro interference studies in three European laboratories on Hitachi instruments. The...

journal_title：Annals of clinical biochemistry

authors： Sonntag O,Scholer A

更新日期：2001-07-01 00:00:00

abstract：:Many contradictory results have been published on the stability of total non-esterified fatty acids in blood, plasma and serum under different storage conditions. The present study was undertaken to investigate the stability of non-esterified fatty acids, measured with an enzymatic method, in samples of EDTA-treated p...

journal_title：Annals of clinical biochemistry

authors： Menéndez LG,Fernández AL,Enguix A,Ciriza C,Amador J

更新日期：2001-05-01 00:00:00

abstract：:The measurement of carbamylated haemoglobin is a useful indicator of uraemic state during the preceding few weeks in patients with renal failure. In diabetic uraemic patients with hyperglycaemia, glycation of haemoglobin may interfere with its carbamylation, as both reactions involve the free amino groups of the prote...

journal_title：Annals of clinical biochemistry

authors： Hammouda AM,Mady GE

更新日期：2001-03-01 00:00:00

abstract：:Four hundred and fifty-four subjects with hyperamylasaemia were screened for the presence of macroamylase using polyethylene glycol precipitation (PEG) and the Beckman automated amylase assay based on the hydrolysis of maltotetraose. Twenty-five subjects (5.5%) exhibited PEG precipitation values suggestive of macroamy...

journal_title：Annals of clinical biochemistry

authors： Lawson GJ

更新日期：2001-01-01 00:00:00

abstract：:A number of cardiac interventional procedures are available for the treatment of angina, including percutaneous transluminal coronary angioplasty (PTCA), stent insertion and rotational atherectomy (RA). Variable degrees of myocardial cell injury during PTCA and stent insertion have been observed, based on rises in cre...

journal_title：Annals of clinical biochemistry

authors： Harris BM,Nageh T,Marsden JT,Thomas MR,Sherwood RA

更新日期：2000-11-01 00:00:00

abstract：:Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure...

journal_title：Annals of clinical biochemistry

authors： Cope G,Nayyar P,Holder R,Brock G,Chapple I

更新日期：2000-09-01 00:00:00

abstract：:We examined the reactivity of human serum albumin-bound bilirubin and its photoisomers as substrates for a direct bilirubin assay using bilirubin oxidase. The reduction of (EZ)-cyclobilirubin reached 100% 5 min after addition of the enzyme at any pH tested (3.5-7.4) in 0.1 mol/L phosphate buffer, whereas the reduction...

journal_title：Annals of clinical biochemistry

authors： Itoh S,Kusaka T,Imai T,Isobe K,Onishi S

更新日期：2000-07-01 00:00:00

abstract：:Measurement of urinary free cortisol using the Bayer Automated Chemiluminescent System (ACS:180 PLUS) was evaluated and compared with an in-house extraction radioimmunoassay (RIA). Inter-assay coefficients of variation were acceptable, being respectively 5.3%, 4.8% and 3.8% at 141, 406 and 942 nmol/L (n=20) for the AC...

journal_title：Annals of clinical biochemistry

authors： Kennedy DM,Selby C,Lawson N

更新日期：2000-07-01 00:00:00

abstract：:A total of 1381 urine specimens were screened using a Microgenics CEDIA urinary primary methadone metabolite (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine; EDDP) immunoassay (cut-off calibrator concentration of 100 microg/L) in combination with a Dade Behring EMIT urinary methadone immunoassay (cut-off calibrator...

journal_title：Annals of clinical biochemistry

authors： George S,Parmar S,Meadway C,Braithwaite RA

更新日期：2000-05-01 00:00:00

abstract：:Auto-antibodies to oxidized low-density lipoprotein (ox-LDL) are thought to play a pivotal role in the pathogenesis of atherosclerosis. This study investigates the value of auto-antibodies to ox-LDL as a predictive marker of atherosclerosis in patients with both homozygous and heterozygous familial hypercholesterolaem...

journal_title：Annals of clinical biochemistry

authors： Paiker JE,Raal FJ,von Arb M

更新日期：2000-03-01 00:00:00

abstract：:Pancreatic elastase 1 (E1), a digestive protease, is synthesized by the acinar cells of the pancreas. Using an enzyme-linked immunosorbent assay, we evaluated stool E1 levels in the following groups of patients. (a) Specimens submitted for occult blood examination from 20 adults, over 3 consecutive days, to assess the...

journal_title：Annals of clinical biochemistry

authors： Phillips IJ,Rowe DJ,Dewar P,Connett GJ

更新日期：1999-11-01 00:00:00

abstract：:Haptoglobin is an acute-phase protein with three major phenotypes: Hp 1-1, Hp 2-1 and Hp 2-2. Studies have shown that functional differences between these phenotypes have important consequences in a number of pathological disorders (e.g. cardiovascular disease, autoimmune disorders, infectious disease), making phenoty...

journal_title：Annals of clinical biochemistry

authors： Wassell J,Keevil B

更新日期：1999-09-01 00:00:00

abstract：:A bloodspot assay for testosterone has been developed using an antiserum raised against testosterone-3-carboxymethyloxime-bovine serum albumin (T-3 CMO-BSA) conjugate and 125I-T-3CMO-histamine tracer. The method has a detection limit of 0.4 nmol/L with between-batch precision of less than 15% for testosterone values b...

journal_title：Annals of clinical biochemistry

authors： Walker J,Hughes IA,Wood PJ

更新日期：1999-07-01 00:00:00

abstract：:The aim of this study was to assess the performance of a commercially available procedure for detecting anti-Sjögren's syndrome A (anti-SSA) and anti-Sjögren's syndrome B (anti-SSB) antibodies by immunoblotting (IB) and compare it with double immunodiffusion (DID). We also studied the clinical significance of these pr...

journal_title：Annals of clinical biochemistry

authors： Yago M,Belmonte MA,Olmos MJ,Beltran J,Teruel C,Segarra M

更新日期：1999-05-01 00:00:00

abstract：:The purpose of this study was to assess retrospectively the prevalence of severe hyponatraemia in a hospital population and its laboratory investigation, treatment and clinical outcome. Over a 6-month period 47 patients (27 women and 20 men) were found to have a plasma sodium concentration of < or = 120 mmol/L (this n...

journal_title：Annals of clinical biochemistry

authors： Crook MA,Velauthar U,Moran L,Griffiths W

更新日期：1999-03-01 00:00:00

abstract：:The effect of oral vitamin E supplementation on the oxido-reductive status of red blood cells in normal mice and those subject to oxidative stress by chronic administration of the anti-tumour drug Adriamycin was investigated. Mice were randomly separated into three groups of 20 animals each and maintained on diets ide...

journal_title：Annals of clinical biochemistry

authors： Thabrew MI,Samarawickrema N,Chandrasena LG,Jayasekera S

更新日期：1999-03-01 00:00:00

abstract：:The effect of acetate and bicarbonate haemodialysis (HD) on the concentrations of erythrocyte, whole-blood and plasma total carnitine (TC), free carnitine (FC), short- (SC) and long-chain acylcarnitine (LC) and acylcarnitine (AC) as well as the ratio of AC to FC was investigated in 30 healthy subjects (15 men and 15 w...

journal_title：Annals of clinical biochemistry

authors： Alhomida AS

更新日期：1999-01-01 00:00:00

abstract：:Antibodies to glutamic acid decarboxylase (GAD) are a useful autoimmune marker for type 1 diabetes mellitus in Caucasians. We examined antibodies to GAD and their relationships with clinical features and pancreatic beta cell function in 140 young Chinese diabetic patients. Over an 18-month period beginning in 1995, 14...

journal_title：Annals of clinical biochemistry

authors： Ko GT,Chan JC,Yeung VT,Chow CC,Li JK,Lau MS,Mackay IR,Rowley MJ,Zimmet P,Cockram CS

更新日期：1998-11-01 00:00:00

abstract：:The effect of progestins on intracellular corticosteroid-binding globulin (CBG) mRNA expression in an endometrial cancer cell line (Ishikawa) was examined in an attempt to understand the biological effects of high-dose progestins in the treatment of well-differentiated uterine endometrial cancers. Oestradiol-17 beta (...

journal_title：Annals of clinical biochemistry

authors： Misao R,Nakanishi Y,Fujimoto J,Tamaya T

更新日期：1998-09-01 00:00:00

abstract：:Study of the glycoprotein hormones, including hCG, is complex and evolving, and has benefited from recent major advances in analytical technology and molecular biology. It is important to be aware of the effect that these technological advances have, both on the analytical and the clinical requirements for provision o...

journal_title：Annals of clinical biochemistry

authors： Sturgeon CM,McAllister EJ

更新日期：1998-07-01 00:00:00

abstract：:Protoporphyrin and zinc-protoporphyrin were measured in the erythrocytes of normal subjects, workers exposed to lead and patients with iron deficiency and erythropoietic protoporphyria (EPP). Results showed significantly higher levels of zinc-protoporphyrin in the lead-exposed workers (P < 0.0001), patients with iron ...

journal_title：Annals of clinical biochemistry

authors： Nelson JC,Westwood M,Allen KR,Newton KE,Barth JH

更新日期：1998-05-01 00:00:00

abstract：:The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines (n = 5) were assayed at 60 degrees and 70 degrees C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient...

journal_title：Annals of clinical biochemistry

authors： Trewick AL

更新日期：1998-03-01 00:00:00

abstract：:We examined the reproducibility of oral glucose tolerance tests (OGTT) using the World Health Organization criterion in 212 Chinese subjects (male 149, female 63) who underwent two 75 g OGTTs within a 6-week period. The overall reproducibility was 65.6% (139/212) of which 74 subjects had normal glucose tolerance, 24 h...

journal_title：Annals of clinical biochemistry

authors： Ko GT,Chan JC,Woo J,Lau E,Yeung VT,Chow CC,Cockram CS

更新日期：1998-01-01 00:00:00

abstract：:Most UK clinical laboratories use alkaline phosphatase (ALP) methods similar to that proposed by the International Federation of Clinical Chemistry (IFCC), based on the use of 2-amino-2-methyl-1-propanol (AMP) buffer. We present evidence of significant differences in results produced by apparently similar commercial A...

journal_title：Annals of clinical biochemistry

authors： Lamb EJ,Browne M,John WG,Price CP

更新日期：1998-01-01 00:00:00

abstract：:We evaluated a novel assay for the measurement of 1,25 dihydroxyvitamin D (1,25 (OH)2D). Immunoextraction of 1,25(OH)2D is performed using a mini column containing a solid-phase monoclonal antibody followed by radioimmunoassay (RIA) using an 125I-labelled 1,25(OH)2D derivative tracer and Sac-cell separation. The mean ...

journal_title：Annals of clinical biochemistry

authors： Fraser WD,Durham BH,Berry JL,Mawer EB

更新日期：1997-11-01 00:00:00

abstract：:The magnitude of the inflammatory response to surgery depends on the degree of injury during surgical procedures. Laparoscopic techniques are generally associated with less postoperative pain and shorter hospital stay compared with open procedures, presumably due to less tissue injury and reduced inflammatory response...

journal_title：Annals of clinical biochemistry

authors： Labib M,Palfrey S,Paniagua E,Callender R

更新日期：1997-09-01 00:00:00

abstract：:We developed a new simple assay for potassium ion in serum using urea amidolyase (UAL) from yeast sp. The method is based on activation of the enzyme by potassium ion. We eliminated endogenous ammonium ion by use of glutamate dehydrogenase (GLDH), and then monitored the production of ammonium ion by UAL, urea, ATP, bi...

journal_title：Annals of clinical biochemistry

authors： Kimura S,Iyama S,Yamaguchi Y,Hayashi S,Fushimi R,Amino N

更新日期：1997-07-01 00:00:00

abstract：:We have studied 46 patients, 30 men and 16 women, with type 2 (non-insulin-dependent) diabetes in a follow-up period of 6-52 months (mean 30 months). The patients were consecutively entered in the study from the out-patient diabetic clinic. None had urinary tract infections nor proteinuria at entry. Investigations wer...

journal_title：Annals of clinical biochemistry

authors： Friis T,Pedersen LR

更新日期：1997-05-01 00:00:00

abstract：:Further data suggesting that assessment of atypicality may be a valuable addition to Down's syndrome screening is presented, based on results from 1521 unaffected pregnancies and 190 chromosomally abnormal pregnancies. For a 1% increase in false positive rates (over the Down's screening-related false positives), it is...

journal_title：Annals of clinical biochemistry

authors： Reynolds TM

更新日期：1997-05-01 00:00:00

abstract：:We assayed interleukin 6 (IL-6) concentrations in cerebrospinal fluid (CSF) from patients affected by meningitis of different aetiologies, and verified whether IL-6 can be used as a diagnostic marker in the differential diagnosis of meningitis. We used a monoclonal antibody enzyme immunoassay to test 98 CSF samples cl...

journal_title：Annals of clinical biochemistry

authors： López-Cortés LF,Cruz-Ruiz M,Gómez-Mateos J,Jiménez-Hernández D,Viciana-Fernández P,Jiménez-Mejías E

更新日期：1997-03-01 00:00:00

abstract：:Using a high-performance liquid chromatography method, we measured seven commonly prescribed benzodiazepines (chlordiazepoxide, nitrazepam, nordiazepam, oxazepam, lorazepam, temazepam and diazepam) in 100 urine samples obtained from patients attending the Leeds Addiction Unit. All of the urines selected for investigat...

journal_title：Annals of clinical biochemistry

authors： Garretty DJ,Wolff K,Hay AW,Raistrick D

更新日期：1997-01-01 00:00:00

abstract：:Eccentric exercise causes release of muscle creatine kinase (CK) 3-4 days after exercise. Racial variation in basal serum CK has been reported but the reasons for this are unknown. We studied 30 subjects of different ethnic origin (Caucasian, Afro-Caribbean, Asian) before and after eccentric exercise. Basal serum CK w...

journal_title：Annals of clinical biochemistry

authors： Sherwood RA,Lambert A,Newham DJ,Wassif WS,Peters TJ

更新日期：1996-07-01 00:00:00

abstract：:We investigated the contributions made by the entero-insular axis, proinsulin and the fractional hepatic extraction of insulin to the hyperinsulinaemia characteristic of polycystic ovarian syndrome (PCOS). We measured plasma glucose, gastric inhibitory polypeptide (GIP), glucagon-like peptide-1 (7-36 amide) (GLP-1(7-3...

journal_title：Annals of clinical biochemistry

authors： Gama R,Norris F,Wright J,Morgan L,Hampton S,Watkins S,Marks V

更新日期：1996-05-01 00:00:00

abstract：:We developed a binary gradient high-performance liquid chromatography (HPLC) method for measuring HbA2 in whole blood samples using a Pharmacia Mono Q column (1 mL) and measurement at 415 nm. The assay requires a simple lysis and centrifugation step before injection onto the column. We found good agreement of results ...

journal_title：Annals of clinical biochemistry

authors： Keevil BG,Maylor PW,Rowlands D

更新日期：1996-05-01 00:00:00

abstract：:We evaluated a new immunoselective enzyme assay of bone-specific alkaline phosphatase (ALP). The monoclonal antibody used in this assay was raised against purified bone-specific ALP obtained from SAOS-2 human osteosarcoma cell line. Calibration was based on the enzyme's own activity. The relative activity of the antib...

journal_title：Annals of clinical biochemistry

authors： Hata K,Tokuhiro H,Nakatsuka K,Miki T,Nishizawa Y,Morii H,Miura M

更新日期：1996-03-01 00:00:00

abstract：:Seventeen laboratories in the British Isles participated in a study to compare six different commercially available immunoassays for serum lipoprotein(a) (Lp(a)) and to establish reasons for the variations in the measurement of serum Lp(a) concentrations. Pooled serum was distributed neat and after dilution at a centr...

journal_title：Annals of clinical biochemistry

authors： Mackness MI,Bhatnagar D,Weiringa G,MBewu A,Haynes B,Durrington PN

更新日期：1996-01-01 00:00:00

abstract：:We studied the serum protein binding of 3H-labelled progesterone, oestradiol and testosterone, and five 125I-labelled analogues of these steroids. All tracers investigated appeared to be bound by proteins in every serum sample tested. The addition of blocking agents caused a substantial reduction in serum protein bind...

journal_title：Annals of clinical biochemistry

authors： Micallef JV,Hayes MM,Latif A,Ahsan R,Sufi SB

更新日期：1995-11-01 00:00:00